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6 years ago in Data Analysis By Gopalakrishna Kaza

Steps included in synthesise recombinant DNA

I want to synthesise recombinant DNA. Can I use enzymes for this purpose? And also mention the steps included in this process

DataAnalysis

All Answers (3 Answers In All)

By Zeba Malik Answered 6 years ago

Yes definitely. Enzymes such as endonucleases, lysing enzymes, cleaving enzymes, synthesizing enzymes and alkaline phosphatases can be used.

By Gopalakrishna Kaza Answered 6 years ago

Zeba, please explain the steps involved in recombinant DNA technology ( brief would do).

By Zeba Malik Answered 6 years ago

Sure.
1)Isolation of DNA segment
The DNA is used as a passenger and is taken out from the cell using suitable enzyme. The DNA is then isolated from the cell contents and are cleaved using endonuclease to have complementary sticky ends .
2) Formation of recombinant DNA
The complementary sticky ends pair and their ends must be sealed with ligase. This generates a recombinant DNA.
3) Production of multiple copies of recombinant DNA
Next produce multiple copies of recombinant DNA.
4) Introduction of recombinant DNA into host
Yeast or bacteria are used as hosts for recombinant DNA. (The bacteria used as hosts should be without plasmids).
5) Screening of the transformed cells
Next step is to investigate which of the cells nurture recombinant DNA molecule that consists of gene of interest. To know more about this process, visit https://www.youtube.com/watch?v=ZW9zPdb_Bs0.

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