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While storing a Western blot membrane in 1x TBST buffer without probing with antibodies temporarily is feasible, researchers should take precautions to maintain the stability and integrity of the protein samples. It is advisable to store the membrane at 4°C or even -20°C to minimize protein degradation and preserve the quality of the transferred proteins. Additionally, researchers may consider adding protease inhibitors to the TBST buffer to prevent proteolytic degradation during storage. However, prolonged storage without antibody probing increases the risk of non-specific binding and background signal in subsequent detection assays. Therefore, researchers should aim to probe the membrane with primary antibodies as soon as possible to minimize potential issues and ensure reliable Western blot results.