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7 years ago in Case Study By Manoj

Best method to amplify piece of DNA

All Answers (3 Answers In All)

By Meera Answered 6 years ago

 You cannot use DNA replication to amplify a piece of DNA. you must use PCR approach for this purpose.
 

By Pragati Answered 6 years ago

You have to go for PCR method. DNA replication method simply copies the DNA.
 

By Meera Answered 6 years ago

The key difference include
1.Type of polymerase: In DNA replication there are many DNA polymerases but in PCR, thermostable DNA polymerases are derived from bacteria or archaea.
2.Length of DNA: In PCR the length of the DNA used is much smaller when compared to that of replication method.
3.Features of polymerase used: High fidelity, speed, and repair are the features required for DNA replication. 
PCR reactions utilises simpler polymerases that doesn’t have rich feature.

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