Ask, Learn and Accelerate in your PhD Research

image Post Your Answer


Seeking recommendation for N-terminal specific Anti-Histidine Antibody


what factors should be considered when selecting an appropriate antibody for this application

Can anyone suggest a reliable N-terminal specific Anti-Histidine Antibody for use in protein detection assays, such as Western blotting or immunohistochemistry, and what factors should be considered when selecting an appropriate antibody for this application

All Answers (1 Answers In All) Post Your Answer

By Sur M Answered 1 month ago

When searching for an N-terminal specific Anti-Histidine Antibody, it's crucial to consider factors such as antibody specificity, sensitivity, and compatibility with the intended application. Several commercial suppliers offer antibodies specifically designed to target the N-terminal region of proteins containing Histidine tags, commonly used in recombinant protein expression systems. Additionally, researchers may explore peer-reviewed literature or consult with colleagues to identify validated antibodies with demonstrated performance in detecting Histidine-tagged proteins. Prioritize antibodies with proven specificity for the N-terminal region and optimize assay conditions to maximize detection sensitivity and minimize non-specific binding


Your Answer


View Related Questions